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Simplify Your Sample Processing, Improve Your Analytical Data Quality

Simplify Your Sample Processing, Improve Your Analytical Data Quality

Lipids, including phospholipids, are abundant in biological samples (serum and plasma, specifically). If not removed, they will co-elute with your analytes of interest and cause ion suppression in mass spectrometry analysis, which will in term affect analytical quality (accuracy and precision). In addition, lipid buildup in a column or another component in the analytical system can cause downtime and increase maintenance cost. The popular method of protein precipitation in preparing biological samples for LC-MS analysis does not remove lipids. By using Captiva EMR-Lipid product, lipids are removed as part of protein precipitation workflow, which streamlines the sample preparation and provides a protein and lipid free sample for downstream analysis.

In this presentation, attendees will learn, how and why lipids interfere with analyte detection and reproducibility from LC/MS and LC/MS/MS analysis when dealing with biological matrices.  A new and highly innovative sample preparation product offers a simple process that can selectively and efficiently remove lipids without affecting analyte recovery, which simplifies sample processing and improves analytical data quality.

Key learning objectives:

  • Understand the potential impact of lipid interference when analyzing serum and plasma by LC/MS and LC/MS/MS techniques
  • Learn about a new technology for high percentage lipid removal with high analyte recovery  
  • See method examples and data for problematic analytes such as THC and THC metabolites, vitamins D2 and D3, and per/polyfluoroalkyl substances (PFASs)  

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