The Complexities of Validating the Cannabis Matrix
A method validation was designed and conducted to verify and extend the scope of the method validation under AOAC Certificate No. 010803, iQ-Check Salmonella II Real-Time PCR, and AOAC Certificate No. 121203, iQ-Check STEC VirX and SerO Real-Time PCR, the performance claim of each is to allow for the detection of Salmonella spp. and Shiga toxin-producing E. coli (STEC) from food samples utilizing real-time PCR technology, respectively.
Specifically, this method validation extends the scope of matrices in the initial method validations to include cannabis flower. In addition, this study evaluates a method modification to the initial method validations by harmonizing the Salmonella spp. and STEC sample enrichment steps, utilizing buffered peptone water for both method sample enrichments.
Confirmation of samples was performed using a modified FDA BAM Salmonella and STEC protocol, including CHROMagar and secondary, selective enrichment comparisons. Where possible, the overall study was designed to incorporate Appendix J: AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces, including data analysis and applied statistical methodology to determine most probable number (MPN), probabilities of detection (POD), and method comparisons.