Investigation of Chocolate Matrix Interference on Cannabinoid Analytes

This presentation will discuss the first known findings of chocolate matrix interference on cannabinoid analytes. Stock solutions of four biogenic cannabinoids (Δ9-THC, CBD, CBN, and CBG) and one synthetic cannabinoid (cannabidiol dimethyl ether) were subjected to milk chocolate, dark chocolate, and cocoa powder. A clear trend of matrix interference was observed, which correlates to several chemical factors. The amount of chocolate present is directly proportional to the degree of matrix interference, which yields lower percent recovery rates for the cannabinoid analyte. Structural features on the cannabinoid analytes are shown to affect matrix interference, as cannabinoids with fewer phenolic −OH groups suffer from increased signal suppression. Additionally, aromatization of the p-menthyl moiety appears to correlate with enhanced matrix effects from chocolate products high in cocoa solids. These findings represent the first known documentation of chocolate matrix interference in cannabinoid analysis, which potentially has broad implications for complex matrix testing in the legal Cannabis industry.