Trilogene Seeds Accelerates Its Cannabis Breeding Using Target Sequencing

Trilogene Seeds, the Colorado-based cannabis genetics company, announces advanced cannabis research. The team is at the validation stage of developing a next-generation sequencing solution for cannabis genetic improvement, with the aim of accurately targeting genes of interest and accelerating breeding for commercial cannabis cultivation.

The diecious nature of cannabis contributes to the high heterozygosity, which makes genetic stabilization for cultivation complicated, time and money consuming. All these bottlenecks have forced the industry to propagate through cloning, leading to a fast spread of pests and diseases across commercial facilities and diminishing genetic diversity.

Cannabis should benefit from the advancements in genomics, transcriptomics, and epigenetics being developed for the past two decades. 

Lead geneticist at Trilogene Seeds, Oussama Badad, said: “When it comes to genetic improvement, cannabis should be treated as corn or soybeans.” 

Universities and private sectors all over the world have conducted genomic research on these crops to get to the yield numbers we observe today, and with the new legislation about cannabis research, it is time for the cannabis research to align with other agricultural commodities.

Trilogene Seeds advanced phase of validation for targeted sequencing using DNA hybridization to retrieve information about 120 genes of interest in the cannabis plant, such as cannabinoids, terpenoids, fatty acids, protein content in seed, and bast fiber. The 12k probes are designed based on the reference genome to retrieve information from the functional part of genes and to retrieve promoter regions to study the Cis element involved in gene expression.

“This panel will allow breeders to make decisions very early in the breeding program without a need to grow to maturity, flower dry and phenotype to select and stabilize varieties,” said Matt Haddad, CEO and founder of Trilogene Seeds. 

This panel could also be used by universities working on allele discovery and genome-wide association studies to elucidate genes and regulatory networks involved in traits of interest.

“We are committed to accelerating the development of this complex plant and will continue to push research forward at our new genomics lab in Illinois. This opportunity at Southern Illinois University’s innovation center will allow us to lean on university expertise and infrastructure to accelerate our genetic development projects,” said Matt Haddad, CEO and founder of Trilogene Seeds.

This article has been republished from materials provided by Trilogene Seeds. Note: material may have been edited for length and content. For further information, please contact the cited source.