Identification of Four THC Stereoisomers Formed During Δ⁹-THC Degradation

As the primary phytocannabinoid associated with psychoactive properties, Δ⁹-tetrahydrocannabinol (Δ⁹-THC) is crucial to cannabis testing. Degradation of Δ⁹-THC, from the processing of inflorescences for cannabis isolates or other products, may lead to isomers that can be misidentified and provide invalid potency claims.

This application note describes a method for the separation of the Δ¹⁰-THC and Δ^6a,^10a-THC isomers using a chiral HPLC stationary phase. The use of a chiral column under normal-phase liquid chromatography conditions provides an analytical method to fully separate these THC isomers for identification and accurate determination of potency.

Download this app note to discover a method that:

• Allows you to differentiate between very similar THC stereoisomers
• Utilizes a chiral HPLC column under normal-phase conditions
• Provides accurate, reliable results for the determination of potency and safety